Journal: Immunology

Article Title: Function and expression of CD1d and invariant natural killer T‐cell receptor in the cotton rat ( Sigmodon hispidus )

doi: 10.1111/imm.12532

Figure Lengend Snippet: WTH‐2 and 1B1 but not WTH‐1 monoclonal CD1d antibodies can be used to stain cotton rat CD1d. Representative data from one of two experiments in total are shown. The biotinylated monoclonal mouse anti‐rat antibodies WTH‐1 and WTH‐2 (each 125 ng/ml), detected with streptavidin‐allophycocyanin, and rat anti‐mouse 1B1 phycoerythrin (2 μg/ml) were used to stain Raji cells transduced with rat, mouse, or cotton rat CD1d. Staining intensities are compared to isotype controls.

Article Snippet: CD1d dimer stainings were carried out as previously described 31 and a biotinylated hamster anti‐mouse CD3 ε antibody (145‐2C11; BD Pharmingen) was used to identify TCR expression of TCR transductants.

Techniques: Staining, Transduction

Journal: Immunology

Article Title: Function and expression of CD1d and invariant natural killer T‐cell receptor in the cotton rat ( Sigmodon hispidus )

doi: 10.1111/imm.12532

Figure Lengend Snippet: Organ‐specific CD1d expression in cotton rat primary cells. (a) Representative data for a total of three experiments are shown. Cells (5 × 105) isolated from mouse tissue or cotton rat tissues were stained with the monoclonal antibody (mAb) WTH‐2 FITC (2 μg/ml) or an isotype control. Geometric means of isotype control (tinted grey) or CD1d staining (black) are indicated in each graph. Geometric means of different expression levels (cotton rat lymph node and spleen) are additionally indicated below the histogram. (b) Primary cells (5 × 105) where stained with biotinylated WTH‐2 (3·6 μg/ml) and antibodies against CD3 (2 μg/ml), MHC class II (5 μg/ml, GαM R‐PE) and cotton rat IgG (10 μg/ml, GαR FITC). WTH‐2 positive cells from cotton rat spleen and lymph node were divided into low and high expression and CD3, MHC class II and cotton rat IgG expression of those two populations are compared in histograms. Representative data for two independent experiments are shown.

Article Snippet: CD1d dimer stainings were carried out as previously described 31 and a biotinylated hamster anti‐mouse CD3 ε antibody (145‐2C11; BD Pharmingen) was used to identify TCR expression of TCR transductants.

Techniques: Expressing, Isolation, Staining